Dr. Prakash Saudagar

Assistant Professor

Department of Biotechnology

National Institute of Technology, Warangal - 506004, Telangana, INDIA

: ps@nitw.ac.in

: 08702452898

Interests: Molecular Cell Biology & Protein Biochemistry Molecular Approach to Infectious Diseases Molecular & Biochemical Parasitology

Research Area

      • Molecular Cell Biology & Protein Biochemistry
      • Molecular Approach to Infectious Diseases


Academics

      • Ph.D. (Biotechnology) - 2013: Indian Institute of Technology  Guwahati (IITG).
      • M. Tech. (Biochem. Engg.& Biotech) - 2009: OUCT, Osmania University.
      • B. Tech. (Biotechnology) 2007: JNTU, Hyderabad.


Positions Held

      • Assistant Professor, Department of Biotechnology, NIT Warangal (September 2013 to till date)


Course Taught Previously

 

Teaching Responsibilities (Current & Past)

      • BT101 Engineering Biology
      • BT252 Cell Biology
      • BT254 Molecular Biology & Genetics Lab
      • BT314 Plant Biotechnology
      • BT306 Genetic Engineering Lab
      • BT403 Bioinformatics Lab
      • BT207 Cell Biology Lab

 

Publications

 

Patents

      • Prakash Saudagar, Vikash Kumar Dubey, Anil Kumar Saikia, and Pipas Saha.  Oxabicyclo derivatives as anti-leishmanial agents. Application No. 863/KOL/2013.

 

Journal Publication Details


27. Sasidharan S,  and Prakash S*. Flavones reversibly inhibit Leishmania donovani tyrosine aminotransferase by binding to the catalytic pocket: An integrated in silico-in vitro approach. International Journal of Biological Macromolecules, (Accepted). (2020) DOI: https://doi.org/10.1016/j.ijbiomac.2020.08.107

26. Kadam A, Sasidharan S,  and Prakash S*. Computational design of a potential multi-epitope subunit vaccine using immunoinformatics to fight Ebola virus. Infection, Genetics, and Evolution, (Accepted). (2020) DOI: https://doi.org/10.1016/j.meegid.2020.104464

25. Sasidharan S,  and Prakash S*. Mapping N- and C-terminals of Leishmania donovani tyrosine aminotransferase by gene truncation strategy: A functional study using in vitro and in silico approaches. Scientific Reports, (Accepted). (2020) DOI: https://doi.org/10.1038/s41598-020-69512-y 

24. Sasidharan S, Chandrabose  Selvaraj, Sanjeev K Singh, Vikash K Dubey, Sachin Kumar, Arsenio M. Fialho, and Prakash S*. Bacterial protein azurin and derived peptides as potential anti-SARS-CoV-2 agents: insights from molecular docking and molecular dynamics simulations. Journal of Biomolecular Structure & Dynamics, (Accepted). (2020) DOI:  http://dx.doi.org/10.1080/07391102.2020.1787864 

23. Raj S, Sasidharan S, Balaji S N, Vikash K Dubey, and Prakash S*.Review on natural products as an alternative to contemporary anti-leishmanial therapeutics. Journal of Proteins and Proteomics, (Accepted). (2020) DOI: 10.1007/s42485-020-00035-w31

22. Raj S, Sasidharan S, Balaji S N, and Prakash S*.An overview of biochemically characterized drug targets in metabolic pathways of Leishmania parasite. Parasitology Research, (Accepted). (2020) DOI: https://doi.org/10.1007/s00436-020-06736-x

21. Sasidharan S, and Prakash S*. Concerted motion of structure and active site charge is required for tyrosine aminotransferase activity in Leishmania parasite. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 232: 118133. (2020) DOI: https://doi.org/10.1016/j.saa.2020.118133

20. Bora N, Sasidharan S, Raj S,  Balaji S N, and Prakash S*. Utilising capsid proteins of poliovirus to design a multi-epitope based subunit vaccine by immunoinformatics approach. Molecular Simulation, 46(5), 419-428. (2020) DOI: https://doi.org/10.1080/08927022.2020.1720916

19. Mittal A, Sasidharan S, Raj S,  Balaji S N, and Prakash S*. Exploring the Zika Genome to Design a Potential Multiepitope Vaccine Using an Immunoinformatics Approach. International Journal of Peptide Research and Therapeutics, 1-10, (2020) DOI: https://doi.org/10.1007/s10989-020-10020-y

18. B Bharath, S Sasidharan, SK Bhamidipati, and Prakash S*. Green-Synthesized FeSO4 Nanoparticles Exhibit Antibacterial and Cytotoxic Activity by DNA Degradation. Current pharmaceutical biotechnology. (Accepted), (2019) DOI: 10.2174/1389201021666200101111643

17. Raj S, Saha G, Sasidharan S, Vikash K Dubey, and Prakash S*. Biochemical characterization and chemical validation of Leishmania MAP Kinase-3 as a potential drug target. Scientific Reports, 9, 16209 (2019). DOI: https://doi.org/10.1038/s41598-019-52774-6

16. Sasidharan S, and Prakash S*. Biochemical and structural characterization of tyrosine aminotransferase suggests broad substrate specificity and a two-state folding mechanism in Leishmania donovaniFEBS open bio, 9(10), 1769-1783. (2019) DOI: 10.1002/2211-5463.12715

15. Raj S, Sasidharan S, Vikash K Dubey, and Prakash S*. Identification of lead molecules against potential drug target protein MAPK4 from L. donovani: An in-silico approach using docking, molecular dynamics and binding free energy calculation. PLoS ONE, 14(8): e0221331. (2019) DOI: https://doi.org/10.1371/journal.pone.0221331

14. Jay P, Yadav S, Saha G,  Chiranjivi A K, Kumar S, Sasidharan S, Prakash S*, and Vikash K Dubey*. Episomal expression of human glutathione reductase (HuGR) in Leishmania sheds light on evolutionary pressure for unique redox metabolism pathway: Impaired stress tolerance ability of Leishmania donovani. International journal of biological macromolecules, 121, 498-507. (2019) (*Corrosponding authors)

13. Mayuri B, Kavitha P,  Raj S, Prakash S, Durgaiah G, Narsimha R Y, and Laxma R K., Novel Schiff based complexes: Synthesis, characterization, DNA binding, DNA cleavage, and molecular docking studies. Journal of the Iranian Chemical Society journal, 15(6), 1377-1389. (2018)

12. Prakash S and Vikash K. Dubey. Molecular Mechanisms of In vitro Betulin Induced Apoptosis of Leishmania donovani. American Journal of Tropical  Medicine and Hygiene. 90, 354-360. (2014)

11. Prakash S and Vikash K. Dubey. Carbon Nanotube Based Betulin Formulation shows better efficacy against Leishmania parasite. Parasitology International, 63, 772-776. (2014)

10. Prakash S, Shyam Lal Mudavath, Pipas Saha, Anil K. Saikia, Shyam Sundar and Vikash Kumar Dubey. In vivo assessment of antileishmanial property of 4-(4,4,8-Trimethyl-7-oxo-3-oxabicyclo[3.3.1]non-2-yl)- benzoic acid methyl ester, an oxabicyclo [3.3.1] nonanones. Letters in Drug Design and Discovery, 11, 937-939. (2014)

9. Saravanan P, Prakash S, Vikash K. Dubey and Sanjukta Patra. Discovery of novel anti-leishmanial agents targeting LdLip3 lipase. Journal of Molecular  Graphics and Modelling. 49, 68-79. (2014)

8. Prakash S, Saha P, Anil K. Saikia and Vikash K. Dubey. Molecular mechanism underlying antileishmanial effect of oxabicyclo[3.3.1]nonanones: Inhibition of key redox enzymes of the pathogen European Journal of Pharmaceutics and Biopharmaceutics. 2013, 85, 569-577. (2013)

7. Prakash S?, Mousumi Das?, Shyam Sunder and Vikash K. Dubey.  Miltefosine unresponsive Leishmania donovani has better ability of resist reactive oxygen    species. FEBS Journal; 280, 4807-4815. (2013) (? Equal contribution).

6. Hazara S, Ghosh S, Sharma MD, Das M, Prakash S, Prajapati V.K, Dubey V.K, Sundar S, Hazra B. Evaluation of a diospyrin derivative as antileishmanial  agent and potential modulators of ornithine decarboxylase of Leishmania donovani. Experimental Parasitology. 135, 407-413. (2013)

5. Abhinav Grover, Sushil K. Shakyawar, Prakash S, Vikash K. Dubey and Sundar Durai. Epitopic  analysis  of potential  vaccine  candidate  in  Leishmania  infantum  for  development  of  human vaccine. Letters in Drug Design & Discovery. 9, 698-705. (2012)

4. Ruchika Bhardwaj, Prakash S and Vikash K. Dubey. Nanobiosciences: A contemporary line for Antiparasitic Drug. Molecular and Cellular Pharmacology. 4, 97-103. (2012)

3. Prakash S and Vikash K. Dubey. Cloning,  expression,  characterization,  and inhibition  studies  on  trypanothione  synthetase,  a  drug  target  enzyme,      from  Leishmania donovani. Biological Chemistry. 392, 1113-1122. (2011)

2. Santhosh K. Venkatesan, Prakash S, Anil K. Shukla and Vikash K. Dubey.  Screening natural products database for identification of potential anti-    leishmanial chemotherapeutic agents. Interdiscip. Sci. Comput. Life Sci. 3, 1-15. (2011)

1. Santhosh K. Venkatesan, Prakash S and Vikash K. Dubey. Identification of novel inhibitors of trypanothione synthetase from two Leishmania species:    Comparative in silico analysis. Journal of Proteins and Proteomics. 2, 41-48. (2011)

PHDs Supervised

 

Ongoing Ph.D. Students

    • Ms. Shweta Raj
    • Mr. Santanu Sasidharan

Workshops/Conferences

 Conference, Seminar & Workshop Conducted:

 

3. National Conference on  "Molecular Biotechnology". Funded by TEQIP-lll, NITW, 4th-5th January 2019. Coordinators: Dr. Prakash Saudagar & Dr. K. Divakar.

 

2. "Continuous Education Program on "Molecular Biotechnology: Tools & Techniques". Funded by TEQIP-lll, NITW, 22th-26th October 2018. Coordinators: Dr. Prakash Saudagar & Dr. K. Divakar.

 

1. Teaching and Learning of Molecular Biology and Enzymology through hands-on Experience. Funded by TLC-NITW, PMMMTNMTT, MHRD, Govt. of India. 18th-23th September 2018. Coordinators: Dr. Prakash Saudagar, Dr. K. Divakar & Dr. R Satish Babu.

 


Conference Proceeding:

 

10. Prakash Saudagar. "Studies on mitogen-activated protein kinase of Leishmania parasite as a therapeutic target, Recent Advancements in Biochemical Engineering &  Biotechnology (RABAB), Indian Institute of Technology BHU (IIT BHU), Varanasi, India, March 15-16, 2019.[Invited talk]

 

9. Prakash Saudagar* and Vikash Kumar Dubey. "Molecular mechanism and increased antileishmanial activity of carbon nanotube based betulin forulation". International Conference on Nanomedicine and Nanobiotechnology-2016 (ICONAN-2016), University Pierre and Marie Curie in Paris, France, September 28-30,2016. [ Poster]

 

8. Prakash Saudagar* and Vikash Kumar Dubey. "Betulin attached to functionalized carbon nanotubes showed better efficacy against Leishmania parasite". 2nd International Conference on Infectious Diseases and Nanomedicine-2015 (ICIDN-2015), Kathmandu, Nepal, December 15-18,2015. [Best poster Award].


7. Prakash Saudagar*. 7th IEEE International Conference on "Technology For Education-2015 (T4E-2015)", held at National Institute of Technology Warangal (NITW), Warangal, India, December 09-12th, 2015.


6. Prakash Saudagar*. Redox Metabolism in Leishmania Parasite. Recent Developments in Medical Biotechnology and Structure Based Drug Designing-2015 (RDMBSBDD-2015), Indian Institute of Technology Guwahati (IITG), Guwahati, India. December 06-07,2015. [Invited talk].


5. Prakash Saudagar and Vikash K. Dubey*. Oxabicyclo derivatives as a new class of antileishmanial agents:target identification and understanding molecular  mechanism of parasite death. International Meeting on Emerging Diseases and Surveillance (IMED 2013). Vienna, Austria. February 15-18, 2013. [DST Travel  Award].


4. Prakash Saudagar and Vikash Kumar Dubey*. Trypanothione Synthetase from L. donovani : Cloning, expression, characterization, and inhibition studies. 80th Annual Meeting of Society of Biological Chemists, held at Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India, November 12-15, 2011. [Best poster Award].


3. Prakash Saudagar and Vikash Kumar Dubey*. Trypanothion synthetase: A validated drug target enzyme from Leishmania donovani. The 18th International  Conference (POST ISCBC-2012) IASST Guwahati, Assam, India. January 28-30, 2012.[ Poster]


2. Vikash Kumar Dubey, Anil Kuamr Shukla and Prakash Saudagar. Current trends in anti-leishmanial drug discovery. International conference on New  Horizones in Biotechnology, Trivandrum, India, November 21-24, 2011. [Invited talk].


1. Prakash Saudagar, Santhosh Kannan Venkatesan and Vikash Kumar Dubey*. Molecular modeling, virtual screening and comparative analysis of. Trypanothione    Synthetase from L. donovani and L. major for the identification of new inhibitors as a drug. International Conference on Frontiers on Biological Sciences. NIT,  Rourkela. October 1-3, 2010. [Oral presentation].

 

Workshop/ STTP

2. Prakash Saudagar*. 7th IEEE International Conference on "Technology For Education-2015 (T4E-2015)", held at National Institute of Technology Warangal (NITW), Warangal, India, December 09-12th, 2015.  


1. Short Term Training Programme on " Computational Drug Design using Molecular Docking and Virtual Screening", VNIT Nagpur, March 12-14th, 2015.  


Projects

 

R & D Projects

 

1.Title: "Identification of novel drug candidates for leishmaniasis: Studies on redox enzymes of the parasite" 

PI: Dr. Prakash Saudagar (PI)

       Funding Agency: Research seed grant, NIT Warangal

       Period of Support: 2014-2016.

 

  2.Title: "Studies on Mitogen-activated protein kinase of Leishmania parasite as a therapeutic target"

      PI: Dr. Prakash Saudagar (PI)

      Funding Agency: SERB (DST, Govt. of India)

      Period of Support: 2015-2018


  3.Title: "Ultrasonic encapsulation of bioactive compounds to deliver in the food matrix"

      PI: Prof. Shirish H Sonawane (PI)

      Co-PI: Dr. Prakash Saudagar

      Funding Agency: DST, Govt. of India, (India-Russia Joint Call)

      Period of Support: 2018-2020


  4.Title: "Characterization and validation of tyrosine aminotransferase from Leishmania donovani as a potential drug target"

      PI: Dr. Prakash Saudagar (PI)

      Funding Agency: SERB (DST, Govt. of India)

      Period of Support: 2019-2022


Awards and Honors

 

 

Awards

      • Young Scientist Award by Telangana Academy of Sciences, TAS, Telangana State, 2018-19
      • Associate Fellow of the Telangana Academy of Sciences, TAS, Telangana State, 2018-19
      • Young Faculty Award by Venus International Foundation, Centre for Advanced Research and Design, in recognition for the research contribution and achivement in the field of Molecular and Biochemical Parasitology. Chennai, 9th July 2016, India.
    •  
      • Best Poster presentation Award during 2nd International Conference on " Infectious Diseases and Nanomedicine-2015 (ICIDN-2015), Kathmandu, Nepal, December 15-18.
    •  
      • International travel award by Department of Science and Technology (DST), Government of India, for attending "International Meeting on Emerging Diseases and Surveillance" (IMED 2013). Vienna, Austria. February 15-18, 2013.
    •  
      • B.S NarasingaRao Best Poster presentation Award during 80th Annual Meeting of Society of Biological Chemists, India held at CMAP, Lucknow, November 12-15, 2011.
    •  
      • MHRD, Govt India Fellowship to carry out Ph.D. at IITG.

 

NCBI Submissions

      • NCBI, GenBankTM accession number JN561695 (Trypanothione synthetase  gene from Leishmania donovani, UR6 strain)
      • NCBI, Gene Expression Omnibus (GEO) accession number GSE45496 (Gene expression profile of miltefosine responsive and unresponsive Leishmania donovani promastigotes


Professional Associations

      • The Society of Biology Chemists, India (SBCI).
      • The Indian Science Congress (ISC), India.
      • The Biotech Research Society, India (BRSI).

Additional Responsibility

 

Additional Responsibility

      • Warden NIT Warangal IFC-B Mess .
      • Departmental Faculty-in-charge for Cell Biology Lab.
      • Departmental Academic Committee UG  (DAC-UG) Member